Modeling of Oligomeric-State Dependent Spectral Heterogeneity in the B875 Light-Harvesting Complex of Rhodobacter sphaeroides by Numerical Simulation
نویسندگان
چکیده
Willem H. J. Westerhuis,† C. Neil Hunter,† Rienk van Grondelle,‡ and Robert A. Niederman*,§ Department of Molecular Biology and Biotechnology, UniVersity of Sheffield, Sheffield S10 2TN, U.K., Department of Biophysics, Faculty of Physics and Astronomy, Free UniVersity, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands, and Department of Molecular Biology and Biochemistry, Rutgers UniVersity, Piscataway, New Jersey 08854-8082
منابع مشابه
5-Aminolevulinic acid availability and control of spectral complex formation in hemA and hemT mutants of Rhodobacter sphaeroides.
In the photosynthetic bacterium Rhodobacter sphaeroides, two genes, hemA and hemT, each encode a distinct 5-aminolevulinic acid (ALA) synthase isozyme (E. L. Neidle and S. Kaplan, J. Bacteriol. 175:2292-2303, 1993). This enzyme catalyzes the first and rate-limiting step in a branched pathway for tetrapyrrole formation, leading to the biosynthesis of hemes, bacteriochlorophylls, and corrinoids. ...
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The light-harvesting complex I (LH-I) of Rhodobacter sphaeroides has been modeled computationally as a hexadecamer of alphabeta-heterodimers, based on a close homology of the heterodimer to that of light-harvesting complex II (LH-II) of Rhodospirillum molischianum. The resulting LH-I structure yields an electron density projection map that is in agreement with an 8.5-A resolution electron micro...
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The relative levels of mRNA for the reaction-centre L and M subunits, B875 (LH1) alpha and beta polypeptides and B800-850 (LH2) alpha and beta polypeptides, have been measured during pigment induction of Rhodobacter sphaeroides. Over the 6 h of the experiment, bacteriochlorophyll levels increased by at least 100-fold. No transcripts for photosynthetic components were detectable at the start of ...
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The effects of an applied electric field on the Qy absorption and fluorescence spectra of three antenna complexes from photosynthetic bacteria have been measured at 77 K: the bacteriochlorophyll a protein (BCP) from Prosthecochloris aestuarii, the B800-850 light-harvesting complex from Rhodobacter sphaeroides and the B875 light-harvesting complex from Rhodobacter capsulatus. For BCP in a g lyce...
متن کاملRegulation of tetrapyrrole synthesis by light in chemostat cultures of Rhodobacter sphaeroides.
Control of bacteriochlorophyll (Bchl), magnesium protoporphyrin monomethyl ester (MgPME), cytochromes, and coproporphyrin by light was studied with chemostat cultures of Rhodobacter sphaeroides growing at a constant dilution rate. By increasing the growth-limiting light energy flux from 10 to 55 W/m2, specific Bchl contents decreased from 19.3 to 7.9 nmol/mg of protein. This was strictly propor...
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تاریخ انتشار 1999